Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis.
نویسندگان
چکیده
Here we demonstrate that flagellar secretion is required for production of secreted lipase activity in the fish pathogen Yersinia ruckeri and that neither of these activities is necessary for virulence in rainbow trout. Our results suggest a possible mechanism for the emergence of nonmotile biotype 2 Y. ruckeri through the mutational loss of flagellar secretion.
منابع مشابه
Detection and identification of virulent Yersinia ruckeri: the causative agent of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) cultured in Fars province, Iran
From the winter 2002 to spring 2006, 126 moribund rainbow trout with clinical signs of external body haemorrhages around and within the oral cavity were sampled from 10 rainbow trout farms situated in the northwest and west of Fars province, Iran and examined for the detection and identification of Yersinia ruckeri, the causative agent of enteric redmouth disease. Fish kidneys were cultured ase...
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The main objective of this study was to evaluate the pathogenicity of isolated Yersinia ruckeri from Caspian trout. Y. ruckeri specimens were isolated from infected rainbow trout farms located in north of Iran. The identification was confirmed by biochemical tests and 16s rRNA gene sequencing. The pathogenicity test was carried out to determine the virulence of the Y. ruckeri by IP injection...
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Yersinia ruckeri is a Gram-negative pathogen that causes enteric redmouth disease in salmonids. A gene from Y. ruckeri encoding an extracellular protease termed yrp1 (Yersinia ruckeri protease 1) was cloned from a Sau3AI library constructed in pUC19 and analysed in gelatin-supplemented medium. The nucleotide sequence of the yrp1 gene indicated an ORF encoding a protein of 477 aa. On the basis o...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 75 20 شماره
صفحات -
تاریخ انتشار 2009